Cultures throughout the world have observed and used many phenomena of the night sky. This often included the grouping of stars into constellations or asterisms. Certain cultures also observed and identified features within the dark areas of the Milky Way where dust and gas block the light of the stars that they obscure.We discuss seven case studies of cultures across various geographical regions that feature dark constellations in their knowledge and traditions: the Maya (Mesoamerica), Inca (South American Andes), Moqoit (Argentina), Tupi (Brasil), Aboriginal and Torres Strait Islanders (Australia), Bugis (Indonesia), Xhosa, Sotho, Zulu, and Tswana (southern Africa), and Polynesians (Pacific)